Journal: Journal of cellular and molecular medicine

Article Title: Omaveloxolone Suppresses Cell Growth and Causes Cell Cycle Arrest by Downregulating CDC20 Expression in Glioblastoma Cells Both In Vitro and In Vivo.

doi: 10.1111/jcmm.70607

Figure Lengend Snippet: FIGURE 6 | Effect of omaveloxolone on cell cycle-related protein expression. Omaveloxolone regulated the expression of cell cycle-related pro- teins in GBM cells. Both cell types were treated with omaveloxolone (0, 600, 800 and 1000 nM) for 24 h. Subsequently, the pellets were collected, and the lysates were isolated using lysis buffer. The results indicated that treatment with omaveloxolone regulated cell cycle-related proteins in GBM8401 and U-87 MG cells. The expression of cyclin D1, CDK4, CDK6, cyclin B1, CDK1 and p21 was analysed using immunoblotting. β-Actin was used as the loading control.

Article Snippet: Propidium iodide (PI) was obtained from Sigma- Aldrich, and the markers were obtained from Bio- Rad Laboratories. β- Actin (1:20,000, Sigma, A5441), cyclin B1, cyclin D1 (1:1000, Proteintech, 55004- 1- AP), CDK4 (1:1000, Sigma- Aldrich, E1Z6R), CDK6 (1:1000, Cell Signalling Technology, D4S8S), p21 (1:1000, Cell Signalling Technology, E2R7A) and CDK1 (1:1000, Cell Signalling Technology, E1Z6R) antibodies were purchased from the corresponding manufacturers.

Techniques: Expressing, Isolation, Lysis, Western Blot, Control